Effect of Chondroitin Sulfates on Athoerosclerosis

Anticoagulant Activities of Chondroitin Sulfates Following Short Term Administration

Abstract

The comparative effect of chondroitin sulfate isomers (CS) on anticoagulation was studied by measuring prothrombin time and thrombelastograph in relation to atherogenesis. The comparison was made between the difference of the isomer, molecular weight and sulfur content of chondroitin sulfate. Electrocardiographic changes by the single intravenous injection of CS to atherosclerotic subjects were also investigated.
i) Prothrombin time was prolonged by addition of CS to the blood in vitro More prolonged prothrombin time was shown in CS-C than in CS-A.
ii) The CS of higher molecular weight (20, 000) prolonged prothrombin time more than the CS of lower molecular weight (4, 000) in CS-C.
iii) Studies on thrombelastogram showed the prolongation of r and K and the shortening of ma and mω by addition of CS in vivo and in vitro. CS-B possesses more marked anti-coagulant effect than CS-C and CS-A.
iv) When CS-C was injected intravenously into rabbits at the rate of 180mg/kg of body weight, the prolongation of r, r+K and the decrease of ma and mε were observed immediately after the administration. These effects showed the maximum at 30-120min and continued 24 hours.
v) In electrocardiogram ST and T depressions were improved in 30% of 32 coronary sclerotic patients following the single intravenous administration of CS-C at 10-20mg/kg of body weight.