Genes & Genetic Systems
Online ISSN : 1880-5779
Print ISSN : 1341-7568
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Putative phospholipid hydroperoxide glutathione peroxidase gene from Arabidopsis thaliana induced by oxidative stress
Manabu SugimotoWataru Sakamoto
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1997 Volume 72 Issue 5 Pages 311-316

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Abstract

An Arabidopsis cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was cloned and sequenced. The cDNA comprised 803 bp and included an open reading frame which encodes a polypeptide of 169 amino acid residues. The deduced amino acid sequence showed about 80 and 50% homology with plant putative PHGPXs and mammalian PHGPXs, respectively. Southern blot analysis suggested that the putative PHGPX gene was a single-copy gene. The expression profile of the putative PHGPX in Arabidopsis under NaCl and Al/Fe treatments, which generate oxidative stress, was analyzed. Northern blot analysis revealed that the Arabidopsis putative PHGPX mRNA levels were increased about 3 and 4.5 times after exposure to NaCl and Al/Fe, respectively. These results suggest that the putative PHGPX gene is induced by oxidative stress in Arabidopsis.

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© 1997 by The Genetics Society of Japan
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