Abstract
Fagopyrum urophyllum is a cross-pollinating perennial woody shrub species belonging to the urophyllum group of Fagopyrum. Natural populations of F. urophyllum were morphologically classified into two distinct groups, the Dali group and the Kunming group without exception. This grouping was verified by molecular phylogeny based on the nucleotide sequence of chloroplast DNA. The reproductive isolation found between the two groups was almost perfect as the distribution of two groups did not overlap each other. The net nucleotide substitution rate (Da) between the two groups was at the same level as between two distinct species. These data suggests that the two groups should be classified into distinct species if future studies on F. urophyllum confirm complete reproductive isolation and no ambiguously classified populations in the border area of the central Yunnan province of China.
