Development and characterization of nuclear microsatellite markers to reveal the neutral demographic background of flower color polymorphism in Geranium thunbergii (Geraniaceae)

Seikan Kurata; Shota Sakaguchi; Hitomi Mishima; Takashi Tsuchimatsu; Motomi Ito

doi:10.1266/ggs.20-00062

This article has now been updated. Please use the final version.

Development and characterization of nuclear microsatellite markers to reveal the neutral demographic background of flower color polymorphism in Geranium thunbergii (Geraniaceae)

Seikan Kurata, Shota Sakaguchi, Hitomi Mishima, Takashi Tsuchimatsu, Motomi Ito

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Keywords: flower color polymorphism, genetic diversity, Geranium thunbergii , microsatellite marker, population genetics

JOURNAL OPEN ACCESS Advance online publication

Article ID: 20-00062

DOI https://doi.org/10.1266/ggs.20-00062

The final version of this article is now available: Vol. 96 (2021), No. 2 pp. 99-104

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Abstract

Nuclear microsatellite markers were developed for Geranium thunbergii, an herbaceous plant characterized by petal color polymorphism. Utilizing RNA sequencing data obtained by next-generation sequencing techniques, we developed and characterized 19 polymorphic microsatellite markers with two to 12 alleles in the nuclear genome. These markers will be used to reveal the genetic structure and demographic history of G. thunbergii in the Japanese archipelago, which will elucidate the genetic background of flower color polymorphism among populations.

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