遺伝学雑誌
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Genetic Studies on Phage DNA Mixed Infection of Purified Phage DNA and Other Active Phage Partcles
Masanaka TERADAIsamu KONDO
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ジャーナル フリー

1959 年 34 巻 8 号 p. 253-260

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抄録
DNA was extracted and purified from the temperate phage a1 liberating from Salmonella paratyphi A 1015.
On the other hand, active phage suspension was prepared with another temperate phage a10s which is serologically unrelated with the above phage and liberate from Salmonella paratyphi A Komatsubara strain.
The mixed infection of S. gallinarum Akita strain with these noninfective a1 phage DNA and active a10s phage suspension was carried out on solid agar medium.
The new type phages just corresponding the recombinant type between both phage strains could be observed among the phage progeny from this mixed infection in proportion of about 10-4-10-5.
So far examined, these new type phages seem to be produced as the result from an interaction between the DNA strand injected from the intact phage a10s and the strand or rather the fragment of a1 DNA somehow entering into the same infected bacterial cells. In addition, one of these new types was revealed to possess the ability of lysogenic conversion concerning salmonella O(I) antigen originally provided in the donor phage of a1 DNA but not in phage a10s.
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© The Genetics Society of Japan
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