Genetical and Biochemical Studies on Amylase in Aspergillus oryzae
1962 年 37 巻 1 号 p. 10-23
詳細
1962 年 37 巻 1 号 p. 10-23
Fifty amylase deficient mutants have been obtained independently from the wild strain of Aspergillus oryzae by using the filtration-concentration technique (eliminating conidia in the minimal solution supplemented with starch or amylopectin as a carb on source) after UV irradiation. These mutants do not grow on the medium containing starch but grow on glucose. Genetic study for amylase production was carried out through the parasexual cycle by using twenty-five mutants.
All twenty-five mutant genes are recessive to normal ones. Amylase production is controlled by genes at two loci; one locus (ae) contains twenty-four alleles (these mutant genes show negative or diminutive in both their dextrinizing power-DP and Saccharifying power-SP) and another locus (ae') includes one allele (the mutant gene shows normal in its DP but not in its SP).
