Abstract
High frequency of transduction of the Gal markers was observed with lysates prepared from strains, Gal- (φ 170dg) (φ 170) and Gal- (φ 170) (φ 170dg). The former was obtained by superinfecting immune syngenote Gal- (φ 170dg) with φ 170, the latter by transduction with Gal- (φ 170) as recipient. The two strains produced active φ 170 and showed the same immunity and segregational pattern.
Doubly lysogenic strains such as Gal- (φ 170dg) (λ), Gal- (φ 170dg) (434) and Gal- (φ 170dg) (φ 80) were isolated by superinfecting immune syngenote Gal- (φ 170dg) with active phages. These strains liberated “active φ 170” phage after UV induction. The proportion of “active φ 170” in these lysates varied considerably and appeared to be determined by the superinfecting phages. Induction of Gal- (φ 170dg) (λ) yielded an Hft lysate.
