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Volume 42, Issue 1
Displaying 1-9 of 9 articles from this issue
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TEIJI IIJIMA1967 Volume 42 Issue 1 Pages 1-10
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSHigh frequency of transduction of the Gal markers was observed with lysates prepared from strains, Gal- (φ 170dg) (φ 170) and Gal- (φ 170) (φ 170dg). The former was obtained by superinfecting immune syngenote Gal- (φ 170dg) with φ 170, the latter by transduction with Gal- (φ 170) as recipient. The two strains produced active φ 170 and showed the same immunity and segregational pattern.
Doubly lysogenic strains such as Gal- (φ 170dg) (λ), Gal- (φ 170dg) (434) and Gal- (φ 170dg) (φ 80) were isolated by superinfecting immune syngenote Gal- (φ 170dg) with active phages. These strains liberated “active φ 170” phage after UV induction. The proportion of “active φ 170” in these lysates varied considerably and appeared to be determined by the superinfecting phages. Induction of Gal- (φ 170dg) (λ) yielded an Hft lysate.View full abstractDownload PDF (903K) -
YASUE FUKUSHI1967 Volume 42 Issue 1 Pages 11-21
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSPreviously we (Seki 1962, Fukushi and Seki 1965) established the relationship between the pupal color of Bombyx mori, Drosophila virilis and Musca domestica and the content of β-alanine in their pupal sheaths. Further information was added to the correlation between color manifestation and β-alanine in the pupal sheaths of insects.
1. In the pupal sheath of the wild type strain of Drosophila melanogaster, β-alanine was found. But β-alanine was absent in pupal sheaths of strains of black adult body color mutants like b and e, although their pupal colors were not black.
2. The content of β-alanine in pupal sheaths of Musca and Drosophila strains, whose adult body colors are paler than those of the wild strains, is higher than in those of the wild type strains.
3. A double mutant, bwb; bp, of M. domestics, shows yellowish-brown body color in the adult and the pupal color is dark black. From the pupal sheath of this strain, a small amount of β-alanine was detected.
4. A phenocopy of the wild type pupal sheath of M. domestica was obtained by rearing larvae of the bp mutant in β-alanine-containing medium at the 4th-day larval stage. Pupal colors of the phenocopy individuals ranged from black to the brown of wild type. A clear correlation was recognized between the grade of color of the phenocopy pupae and the content of β-alanine in the pupal sheaths.
5. By means of reflux and proteinase digestion, a small amount of β-alanine was liberated from the pupal sheath of the wild type strain, but its solubilization behavior was different from that of other amino acids.
6. In the wild type strain of Musca, free β-alanine in the body fluid rapidly increased at the very young pupal stage, but such a phenomenon was not observed in the bp mutant.View full abstractDownload PDF (1048K) -
DAIGORO MORIWAKI, IZUO TOBARI, SHIGERU OHBA, OSAMU KITAGAWA, YOSHIKO N ...1967 Volume 42 Issue 1 Pages 23-38
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe present study was performed in order to investigate the sex-ratio changes in the progeny from males irradiated at different germ cell stages in Drosophila melanogaster.
First, the result indicates that the sex-ratio (_??_/_??_) shifts to the lowest level when the progeny come from males of 6-8 days after irradiation, and recovers to the control level in the progeny from males of 14-16 days; and that the sex-ratio may be reduced with increasing dosage. In addition, it is noticeable that non-irradiated younger fathers produce more sons than daughters, a tendency which decreases with age.
In the second place, the sex-ratio shift changed according to the frequency changes of both induced dominant lethals and elimination of sex-chromosomes, showing a negative correlation, and all the frequencies coincidentally reached a peak on the 7th-8th days after irradiation. The stage-frequency relationship for the sex-chromosome elimination is fundamentally the same as that for the dominant lethals, suggesting that the two phenomena might probably occur through the same mechanism.
Next, in the present study the highest sterility was found during the 6th-8th days. All accounts suggest that sperm utilized during these days represents cells irradiated during the meiotic stages.
Lastly, the proportion of X chromosome elimination (Se) and X chromosome dominant lethals (Sx) to the total shift of the sex-ratio (S) was estimated in each of the three different germ cell stages, i.e., post-meiosis, meiosis, and pre-meiosis. So far as the present data are concerned, the contribution of Se and Sx to S are nearly the same at the meiotic stages, while at the other stages Sx plays a greater part than Se.View full abstractDownload PDF (1482K) -
G. E. NASRAT1967 Volume 42 Issue 1 Pages 39-42
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSTreatment with gamma radiation in combination with malefic hydrazide exhibit an additive effect as regards as the formation of sex-linked recessive lethal mutations, when applied to Drosophila melanogaster spermatozoa.View full abstractDownload PDF (326K) -
TATSUO ISHIKAWA1967 Volume 42 Issue 1 Pages 43-50
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe distribution of mutations on the fine structure map at the ad-8 locus in Neurospora crassa has been analysed. Multiple occurrences of independent mutations at particular sites (hot spots) have been confirmed for mutants of spontaneous origin and ones induced by bromodeoxyuridine and/or fluorodeoxyuridine and by ultraviolet irradiation. Spontaneous and X-ray-induced mutations were characterized by the frequent occurrence of multisite mutations. Discussions have been made on the possible mechanisms of mutations in comparison with some conclusions obtained in bacteriophage T4.View full abstractDownload PDF (681K) -
III. IMMUNOELECTROPHORETIC COMPARISON OF THE SERA OF DOMESTIC FOWL, GUINEA FOWL AND THEIR HYBRIDSTYUBUN SATO, TSUTOMU ISHII, YASOKAZU HIRAI1967 Volume 42 Issue 1 Pages 51-59
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSBy means of electrophoresis, sera of domestic fowls, guinea fowls and their hybrids were fractionized. And their immunoelectrophoretic patterns were used as the standard criteria for the interspecific comparisons. The authors were able to confirm the following results, and they presented some genetic considerations.
1. The concentration of serum proteins of 53.5±6.4mg/ml was estimated in domestic fowls and also that of 37.0±1.4mg/ml was estimated in guinea fowls.
2. Serum of domestic fowls was separated into 12 fractions. Excepting the fractions in α-region and γ-region, the other fractions in domestic fowl serum were in common with those in guinea fowl serum. And the components which exhibited the uncommon characteristics in α-region and γ-region were identified as α1-lipoprotein and immunoglobulin-G (IgG) respectively.
3. The species difference in albumin is due to the difference of electrophoretic migrating rate of this component, i.e., the rate in domestic fowls surpassed that in guinea fowls.
4. Two precipitin lines of albumin were observed in electrophoretic pattern of serum of the genus hybrids. Among them, the line migrated with a surpassing rate corresponds to the albumin in domestic fowls, and the other line migrated with an inferior rate corresponds to that in guinea fowls. And the authors assumed that these three albumin phenotypes are controlled by a pair of codominant allele.
5. The precipitin line of α1-lipoprotein in domestic fowls was observed as a single line, while that in guinea fowls was observed as a compound line. And that in the hybrids was a single line and rather the same as that in domestic fowls. The authors assumed that this interspecific difference is due to the difference of the antigenic construction of α1-lipoprotein.
6. The precipitin line of IgG showed a remarkable specific difference between two species concerned. And in their hybrids, the precipitation of IgG exihibited an intermediate characteristics. The authors consider that the interspecific difference of IgG is also due to the difference of the antigenic construction of this component.View full abstractDownload PDF (1528K) -
TATSUO OMURA1967 Volume 42 Issue 1 Pages 61-65
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSBy using a new method modifying Owen's one, number (one or two) of the Z-chromosome was mainly investigated in the primitive blood corpuscles sucked up from the vitelline vessels of the 6-8 days' chick embryos. The Z-chromosome showed its constant number and structure in one and the same embryo. From the embryological standpoint it is doubtless that the Z-chromosome constitution of the blood corpuscles under investigation will accord with that of the chick embryo itself.View full abstractDownload PDF (1463K) -
I-MIN LIU, HAJIME MATSUURA1967 Volume 42 Issue 1 Pages 67-74
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSAs the result of a low-temperature treatment (0°C) of Trillium kamtschaticum through full cycle of meiosis, the following facts were obtained.
Interstitial chiasma frequency was seen to increase remarkably at metaphase I, and the rate of increase was in proportion to the arm length among individual chromosome arms.
As for the chromosomal aberrations observed at metaphase I, there was a frequent appearance of univalents, fragments and chiasma breakages. The bridges appeared at anaphase I were apparently caused by chiasma breakages.
The increase of interstitial chiasmata, and the relationships between the frequency of chiasma breakages and that of the appearance of bridges are explained on the basis of Matsuura's Neo-two-plane theory.View full abstractDownload PDF (2556K) -
HIDEO KIKKAWA1967 Volume 42 Issue 1 Pages 75-77
Published: 1967
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (284K)
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