GOLGI APPARATUS AND LYSOSOMAL GRANULES OBSERVED IN SOME CULTURED CELLS
1967 年 42 巻 4 号 p. 263-270
詳細
1967 年 42 巻 4 号 p. 263-270
The Golgi apparatus and the lysosomes were demonstrated in human fibroblasts, HeLa cells, mouse embryonic cells, rat fibroblasts, and monkey kidney cells, which were all grown in culture. The Golgi elements were generally detected as the precipitation of a reaction product from thiamine pyrophosphatase (TPPase) activity and the lysosomes were revealed as the granules positive to acid phosphatase (ACPase) activity. (The enzymic activity tests were made on cells seeded on coverslips and fixed by means of a freezing substitution technique.) ACPase activity positive granules showed a strong affinity to neutral red, and appeared with reddish orange fluorescence after acridine orange staining. Rat fibroblasts were exceptional by showing the ACPase activity only. It was shown that ACPase and TPPase activities were specific to each cell organelle, and the topographical relationships suggested the occurrence of certain physiological significance between these organelles.
