Abstract
Subchromatid-like structures were observed in metaphase chromosomes from mouse cells of primary cultures, HeLa cells and FL cells. The chromosome slides prepared by the routine air drying technique were stained with Giemsa (in Sörensen buffer, pH 6.8) after a treatment with one of saline solutions (2×SSC, Sörensen buffer, or Michaelis buffer, pH 6.8) at 60°C or 69°C for appropriate times (1 to 180 minutes). The double strandedness was especially clear at the heavily stained regions in a chromatid which corresponded to bands.
