1976 年 51 巻 2 号 p. 79-89
No obvious difference was noted in the composition of pteridine granule membrane proteins among several oily larval mutants. The protein content of the membranes was 61-68%, the lipid content 29-33%, and the carbohydrate content 0.2-1.1%. The amino acid composition of the membrane structural protein obtained from normal larvae was very similar to that from oily larval mutants.
SDS-urea polyacrylamide gel electrophoresis of the SDS-urea-treated granule membrane proteins obtained from several oily larval mutants revealed multiple protein bands, unlike that of the granule membrane protein from normal larvae. Moreover, these electrophoretic patterns showed significant differences among several oily larval mutants. The cause of this electrophoretic variation of the membrane proteins (as well as lipid and carbohydrate) from oily larval mutants is discussed.
An ultracentrifugal examination of granule membrane protein obtained from the woh mutant demonstrated its heterogeneity. Immunological differences found among granule membrane proteins of w3, wa and wb by the Ouchterlony double diffusion test suggest that the variation is due to some genetic changes in the primary structure of the membrane protein. It is therefore proposed that the structural gene for the membrane protein subunit of pteridine granules may be located at the w3 locus on the 10th chromosome.
