抄録
The esterase isozymes and protein profiles of the diploid and corresponding autotetraploid strains of some wild and cultivated plants were investigated using the gel isoelectrofocusing method.
The esterase zymogram changed with the age of seedlings. The diploid and autotetraploid strains of Oryza sativa cv. Shinriki-mochi had the same zymogram in one day-old seedlings, but their zymograms showed clear differences in older seedlings.
Comparison of the diploid and corresponding autotetraploid strains revealed that three different relationships exist among the zymograms. In the first group, the diploids and autotetraploids had the same pI bands with the same activities. In the second they had the same pI bands, but the activities of some bands were higher in the autotetraploids than in the diploids. In the third group the diploids and autotetraploids had some bands of different pI values. Frequencies of the strains belonging to the three groups were 35, 50 and 15%, respectively.
In the protein profile, the same relationships were found in the diploids and their autotetraploids, as were found in the esterase zymogram. The results demonstrate that the duplication of chromosomes by autotetraploidy brought about quantitative and, in certain instances, qualitative differences in the esterase zymogram and protein profile.
