Abstract
Five enzyme systems, NADP-dependent isocitrate dehydrogenase (Idh), phosphoglucomutase (Pgm), aldehyde oxidase (Aldox), malic enzyme (Men) and aldolase (Ald), were mapped on the third chromosome of Drosophila simulans. Idh and Pgm were mapped at 6.4 and 38.1 on the left arm, respectively. Aldox, Men and Ald were localized at 75.4, 87.7 and 124.6 on the right arm, respectively. On the basis of these findings, together with new mapping data of Idh and Est-C in D. melanogaster, the comparative genetics of the third chromosome of these two species were discussed.