By using starch gel electrophoresis, the zymograms of acid phosphatase and peroxidase in the leaf extracts were examined, and the phylogenetic relationships between diploid parental species, their F
1 plants, synthesized amphiploids, and natural tetraploid species belonging to
Oryza officinalis complex were postulated on the basis of comparisons of zymograms. The similarity index (
s-value; Sheen 1972) was computed in all possible combinations of 17 strains tested. The
s-values found between the tetraploid varieties of
O. punctata (W1024 and W1145) on the one hand and synthesized amphiploids 4x-3 (
punctata×collina), 4x-1137 (intermediate×
punctata), and 4x-1(
punctata×
eichingeri), O. minuta (W0016) and
O. malampuzhaensis (W1159) on the other were 91%, 96%, 48%, 31-36% and 52%, respectively. The tetraploid
punctata strains thus showed much closer relationships with the synthesized amphiploids than with other tetraploid species,
i.e., O. minuta and
O. malampuzhaensis. This suggests that the progenitor of
O.
eichingeri and an intermediate
punctata-eichingeri form would also be the progenitor of species with the C genome which was a component of tetraploid strains of
O. punctata (BBCC).
View full abstract