MOLECULAR CLONING OF AN EcoRI FRAGMENT WHICH CONTAINS 4.5S AND 5S RNA GENES IN TOBACCO CHLOROPLASTS

Abstract

A 0.9×10⁶ dalton EcoRI fragment of tobacco chloroplast DNA has been cloned in E. coli plasmid pMB9. This fragment contains the chloroplast 4.5S and 5S ribosomal RNA genes. A physical map of the recombinant plasmid was constructed by cleavage with several restriction endonucleases and Southern hybridization with ³²P-labeled 4.5S and 5S RNAs.