抄録
In the developmental analyses of Drosophila mutants, it is necessary to distinguish mutant embryos from heterozygous or normal ones.
We have developed an enzyme marker system by means of an X-linked mutation Zwischenfermentn (Zwn). By histochemical staining for the activity of glucose- 6-phosphate dehydrogenase (G6PD, E.C.1.1.1.49) in a single embryo culture, Zwn cultures were distinguishable from Zw+ ones.
The applicability of the method to the analysis of the X-linked developmental lethals was demonstrated by examining the cell differentiation in vitro of an embryonic lethal mutation l(1)EI43. A morphological abnormality was found specifically in the cultures from hemizygous lethal embryos.
