Genetic analysis of very fast sedimenting DNA of bacteriohage T4
1985 年 60 巻 1 号 p. 1-9
詳細
1985 年 60 巻 1 号 p. 1-9
We have previously shown that abnormally fast sedimenting DNA which accumulates upon infection of mutants of T4 phage defective in gene 49 is recombination intermediates, while the mutant gives rise to the recombination deficient phenotype, judging from genetic mating tests under the semipermissible condition (Minagawa and Ryo (1979) Mol, gen. Genet. 170: 113; Minagawa et al. (1983a) Virology 126:138; Miyazaki et al. (1983) Genetics 104:1). To see the step to which the recombination process has proceeded in the DNA, we investigated by the transfection assay whether the generation of contiguous recombinant strands is completed. Such strands were found to be formed normally or nearly normally in the absence of the gene 49 function, supporting the view that the gene product is involved in resolution of branched recombinational intermediates into linear duplexes.
