Insertional mutagenesis in Drosophila

I. Efficiency of transformation and induction of mutations in Drosophila melanogaster

Abstract

A P-element-derived pUChsneo vector was used for the Insertional mutagenesis in D. melanogaster and 152 isogenic lines of the transformants were established. The chromosomal locations of the inserted vectors were examined by genetic analysis conducted in conjunction with neomycin selection. nsertion of the vector in the X, second, and third chromosomes occurred in 24, 42, and 67 lines, respectively. In the other 19 lines, the vector appeared to be inserted in the fourth chromosome or in two or more chromosomes.
From the 152 lines, 11 lethal and 11 sterile mutant lines were isolated. Thus, the usefulness of the intertional mutagenesis in D. melanogaster is demonstrated by this high frequency of mutation induction.