The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 62, Issue 1
Displaying 1-7 of 7 articles from this issue
  • Hiroshi ENDOH
    1987 Volume 62 Issue 1 Pages 21-25
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Interspecific conjugation was induced artificially between the distantly related species belonging to the two different morphological groups, ‘aurelia’ and ‘bursaria’ groups, of Paramecium. Cytological observations showed that the interspecific conjugants underwent normal nuclear changes, including meiosis. However, nuclear changes of co-conjugant cells did not synchronize each other. These results suggest that conjugation in Paramecium involves non-species-specific interactions and its interactions are sufficient for nuclear activation.
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  • Naoto HANZAWA, Hiromichi YONEKAWA, Ken-ichi NUMACHI
    1987 Volume 62 Issue 1 Pages 27-38
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Intra- and inter-populational variations of Japanese dace Tribolodon hakonensis collected at three locations which were geographically distant from each other were examined with restriction fragment analysis of mitochondrial DNA (mtDNA). Twelve mtDNA types (Types I-XII) were discriminated and each type was population specific. Namely, Otsuchi Bay Population contained Types I to V, the Monobe River Population contained Types VI and VII, and Lake Biwa Population contained Types VIII to XII. The mean number of nucleotide differences per site within a population was estimated to be 0.07-0.23%, whereas interpopulational divergence was estimated to be 0.77-0.94%. The present study on mtDNA suggests that the three populations of T. hakonensis have been highly differentiated from each other.
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  • Hideki FUKATA, Hirosuke FUKASAWA
    1987 Volume 62 Issue 1 Pages 39-43
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    The effects of DNA topoisomerases isolated from cauliflower inflorescence on in vitro RNA synthesis have been investigated using Escherichia coli RNA polymerase. When supercoiled pBR322 and pCaMV-S DNAs were used as the template, RNA synthesizing activity was fairly reduced by an addition of DNA topoisomerases. Whereas, a remarkable stimulation of RNA synthesis was observed when isolated cauliflower chromatin was used as the template by means of an addition of DNA topoisomerases.
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  • I. Efficiency of transformation and induction of mutations in Drosophila melanogaster
    Ryu UEDA, Manabu TAKAHISA, Shin TOGASH, Setsu MARUYAMA, Tadashi MIYAKE
    1987 Volume 62 Issue 1 Pages 45-50
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    A P-element-derived pUChsneo vector was used for the Insertional mutagenesis in D. melanogaster and 152 isogenic lines of the transformants were established. The chromosomal locations of the inserted vectors were examined by genetic analysis conducted in conjunction with neomycin selection. nsertion of the vector in the X, second, and third chromosomes occurred in 24, 42, and 67 lines, respectively. In the other 19 lines, the vector appeared to be inserted in the fourth chromosome or in two or more chromosomes.
    From the 152 lines, 11 lethal and 11 sterile mutant lines were isolated. Thus, the usefulness of the intertional mutagenesis in D. melanogaster is demonstrated by this high frequency of mutation induction.
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  • Dong-Sang SUH, Terumi MUKAI
    1987 Volume 62 Issue 1 Pages 51-58
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Recessive lethal mutations were induced at an extremly high rate in the process of extracting the second chromosomes of D. melanogaster sampled from the Ishigakijima population. They were extracted by using the marked inversion stock (Cy/Pm) carrying the M type cytoplasm of the P-M hybrid dysgenic system. The mutation rate was estimated to be 0.090 per second chromosome per generation. This estimate is more than 18 times larger than the ordinary spontaneous mutation rate. The allelism rate between lines of lethal-carrying chromosomes was estimated to be 0.0012 and the average of the allelism rates within lines was estimated to be 0.43. Thus, it was concluded that the mosaic lethal mutations were induced during the spermatogenesis. Its erects on the estimates of some population-genetic parameters were discussed.
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  • II. Phenotypic, genetic and environmental correlations.
    Yoshinari TANAKA
    1987 Volume 62 Issue 1 Pages 59-67
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Genetic correlation structure among several morphological characters, which might have ecological significance (pupal weight, body weight, forewing length, median cell length, hindwing length), of cabbage white butterflies Pieris rapae crucivora have been surveyed by means of statistical genetic analysis. Slightly to highly positive genetic correlations (min=0.04, max=0.87) were estimated among those characters. Principal component analysis, which was undertaken for the phenotypic, the genetic and the environmental correlation matrices each, has extracted first two significant components that may be interpreted as "general size factor (PCI)" and "shape factor (PC2)", which would determine the proportion between body size and wing size, both from the phenotypic and the genetic correlation matrices. There was observed a tendency that the phenotypic and the genetic correlations should resemble each other (the vector correlation of factor loadings in principal component scores is 0.85), the genetic correlations might be availably inferred from the phenotypic correlations at least for signs and relative magnitudes of the coefficients.
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  • Hiroshi SUGE
    1987 Volume 62 Issue 1 Pages 69-80
    Published: 1987
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    The inheritance of elongating ability under submergence in floating rice was studied using a cross Tan-ginbozu×Aswina: Tan-ginbozu is a mutant deficient in gibberellins and Aswina is a common floating variety of rice from Bangladesh. Internodal elongation of F1 plants under submergence showed incomplete dominance. Ability of internodal elongation can be explained in terms of complementary genes, one controls gibberellin production and the other controls responsiveness to ethylene.
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