Abstract
Glycolytic enzyme activities of the two acetate-requiring mutant strains, ace-1 and ace-5, were not so different from the wild type strain as to explain their acetate requirements. Accumulation of glycolytic intermediates coincided with these data. While the wild type strain grows well on glycerol, the growth of the ace-1 and ace-5 strains did not increase by adding glycerol over the level of that in a medium containing acetate. Coincidentally with the inability to use glycerol as a carbon source, these strains did not induce glycerol kinase even in the presence of glycerol. These strains, however, were able to induce this enzyme after incubation at 5°C for 10h even in the absence of glycerol. This means that the glycerol kinase gene per se is normal, but expression of this enzyme in the presence of glycerol is impaired in these strains. Inability of the ace-1 and ace-5 mutants to utilize glycerol does not explain directly the acetate requirement of these strains, but there may be some relationship between them.
