Location of gracile axonal dystrophy (gad) on chromosome 5 of the mouse

Abstract

Linkage tests of gracile axonal dystrophy (gad), an autosomal neurological mutation in the mouse, have shown that gad was located on chromosome 5 and linked to Pgm-1 and W. On the basis of the backcross data, the following recombination frequencies were calculated: Pgm-1-gad, 3.0±1.0 percent; W-gad, 5.4±2.6 percent. Therefore, the gene order was estimated to be centromere-gad-Pgm-1-W. Since Pgm-1 was closely linked to gad, Pgm-1 is a useful marker to recognize homozygotes (gad/gad) before onset of clinical signs and detect carriers (gad/+).