Abstract
Whole plants were obtained efficiently from protoplasts of calluses which were induced from diploid seed (2n=24) and then sub-cultured on MS solid medium for 7-10 months. Agronomic characters and chromosome number were studied in the regenerated plants. Wide variation of agronomic characters such as plant height and heading date was found among the regenerated plants cultivated in the field. In 7 plants in which the chromosome number was determined, 4 tetraploids (2n=48), 2 aneuploids (2n=46) and 1 diploid were observed. The chromosome number was different between plants regenerated from a single protoplast clone, i.e. one was tetraploid (2n=48) and the other was aneuploid (2n=46).
