The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Differential amphiplasty in meiosis of F1 hybrid of Crepis capillaris×C. vesicaria ssp. taraxacifolia by means of silver staining
Hiroko IKEDA-KOMATSU
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1989 Volume 64 Issue 1 Pages 1-8

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Abstract
In F1 hybrid of Crepis capillaris×C. vesicaria ssp. taraxacifolia, somatic cells and PMCs were stained with AgNO3 under the conditions of differential amphiplasty. In the parental plants, 4v chromosome of vesicaria was found to be stained in its secondary constricted region, but it was not the case in F1 hybrid. Before diakinesis of PMC, only one nucleolus was observed. At diakinesis, two nucleoli were observed in 11 out of 50 cells. At prohase II, 2c and 4v chromosomes individually attached to the nucleoli in 40% cells, and at metaphase II in 44% cells. At anaphase in meiosis II, at the maximum four nucleoli were observed in each PMC. It is concluded that the differential amphiplasty in 4v chromosome gradually recovers from diakinesis.
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© The Genetics Society of Japan
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