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Volume 64, Issue 1
Displaying 1-6 of 6 articles from this issue
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Hiroko IKEDA-KOMATSU1989 Volume 64 Issue 1 Pages 1-8
Published: 1989
Released on J-STAGE: July 25, 2006
JOURNAL FREE ACCESSIn F1 hybrid of Crepis capillaris×C. vesicaria ssp. taraxacifolia, somatic cells and PMCs were stained with AgNO3 under the conditions of differential amphiplasty. In the parental plants, 4v chromosome of vesicaria was found to be stained in its secondary constricted region, but it was not the case in F1 hybrid. Before diakinesis of PMC, only one nucleolus was observed. At diakinesis, two nucleoli were observed in 11 out of 50 cells. At prohase II, 2c and 4v chromosomes individually attached to the nucleoli in 40% cells, and at metaphase II in 44% cells. At anaphase in meiosis II, at the maximum four nucleoli were observed in each PMC. It is concluded that the differential amphiplasty in 4v chromosome gradually recovers from diakinesis.View full abstractDownload PDF (1437K) -
Hiroko IKEDA-KMATSU1989 Volume 64 Issue 1 Pages 9-16
Published: 1989
Released on J-STAGE: July 25, 2006
JOURNAL FREE ACCESSA study was undertaken to determine the relationship between differential amphiplasty and chromosome complements in hybrid offspring of C. capillaris×C. vesicaria ssp. taraxacifolia. In cells of offspring plants with somatic chromosome complement of capillaris and vesicaria, the satellite of 4v chromosome of vesicaria disappeared. In a plant without 1c chromosome of capillaris (2n=10=2c+3c+1v+1v+2v+2v+3v+3v+4v+4v), disappearance of the satellite of 4v chromosome was observed. The satellite of 4v chromosomes which had disappeared in F1 hybrid reappeared in the B1 offspring by eliminating the chromosomes of capillaris. Further, it was confirmed that differential amphiplasty recovered under the condition of nucleus substitution. As the result, 2c or 3c chromosome of capillaris may have induced differential amphiplasty.View full abstractDownload PDF (894K) -
Kendo TSUNO1989 Volume 64 Issue 1 Pages 17-26
Published: 1989
Released on J-STAGE: July 25, 2006
JOURNAL FREE ACCESSRelationships between the occurrence frequencies of intragenic recombinants and the allele frequencies at the Est-α locus were examined in two ecological populations of Drosophila virilis. The effective occurrence rate of recombinant alleles is on the average 0.95×10-5. The effects of the recombination were slightly recognized in terms of the allele frequencies.View full abstractDownload PDF (975K) -
Toshiaki KAMEYA, Hiroyuki KANZAKI, Seiichi TOKI, Tomoko ABE1989 Volume 64 Issue 1 Pages 27-34
Published: 1989
Released on J-STAGE: July 25, 2006
JOURNAL FREE ACCESSIntergeneric hybrid plants were obtained through protoplast fusion between red cabbage (Brassica oleracea L.) and radish (Raphanus sativus L.). Protoplast fusion was carried out by the dextran method using protoplasts from hypocotyls and cotyledons of both cabbage and radish. The selection of somatic hybrids utilized the inactivation of cabbage protoplasts with iodoacetamide and the low cell-division ability of radish protoplasts. Ten plants were regenerated from selected colonies. Two of them developed to the flowering stage and formed male sterile flowers, which showed cytoplasmic inheritance. The plant and leaf morphology, chromosome number, acid phosphatase and peroxidase isozyme patterns, sexual compatibility, and SmaI cleavage patterns of chloroplast DNA indicated that these plants were intergeneric hybrids having the nucleus of cabbage and chloroplasts of radish.View full abstractDownload PDF (2084K) -
K. MURAI, Naiyu XU, K. TSUNEWAKI1989 Volume 64 Issue 1 Pages 35-47
Published: 1989
Released on J-STAGE: July 25, 2006
JOURNAL FREE ACCESSIn order to obtain new information on interspecific relationships in the genus Secale, and the phylogenetic relation of Secale to two related genera, Triticum and Aegilops, a restriction endonuclease analysis of chloroplast (ct) DNAs isolated from five Secale species was conducted. First, ctDNA of S. cereale was analyzed using eight restriction endonucleases, and its physical map was constructed by referring to that of T. aestivum. Next, ctDNAs of all five Secale species were analyzed using three endonucleases, BamHI, PstI and SmaI. The chloroplast genome size of all the species was estimated to be about 136kbp, which is very close to those of Triticum and Aegilops. The ctDNA of all the species but S. silvestre produced identical restriction fragment patterns with all the enzymes employed, suggesting early separation of S. silvestre from the rest of species. The patterns of S. silvestre showed one to two fragment differences from those of the four other Secale species. The chloroplast genomes of S. cereale (and other species) and S. silvestre differed by at least three mutations and were designated S1 and S2, respectively. Both chloroplast genomes were closely related to those of Triticum and Aegilops. The magnitude of the chloroplast genome diversification in Secale was almost the same as in Triticum, Aegilops, Hordeum and Avena.View full abstractDownload PDF (1237K) -
Wataru SAKAMOTO, Masaki MOMOSE, Nobuhiro TSUTSUMI, Shigemitsu TANO, Hi ...1989 Volume 64 Issue 1 Pages 49-56
Published: 1989
Released on J-STAGE: July 25, 2006
JOURNAL FREE ACCESSMitochondrial DNAs (mtDNAs) were isolated from several rice strains of Chinsurah boro II (fertile), Zhen Shan 97A (male sterile), Zhen Shan 97 (fertile), and Akihikari (normal japonica). Small plasmid-like DNAs, B1 and B2, which were found firstly in BT line, were cloned into plasmid vectors and mapped in terms of some of restriction enzymes. Plasmid-like DNAs were also observed in the mitochondria of Zhen Shan 97A and Zhen Shan 97 by agarose gel electrophoresis. To examine whether these plasmids were identical or not, hybridization analyses were carried out with B1 and B2 as probes. Consequently, it was suggested that two plasmids of Zhen Shan 97A were identical to B1 and B2, and one of the plasmids of Zhen Shan 97 was identical to B2. The smaller plasmid of Zhen Shan 97 had no homology to either B1 or B2. Co-existence of B1 and B2, while common, is not an obligatory feature. Because B2 was found in the fertile cytoplasm of Zhen Shan 97, an involvement of B2 with cytoplasmic male sterility (cms) might be denied.View full abstractDownload PDF (1261K)
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