Abstract
Shoot primordia were induced from suspended cells derived from Crepis capillaris calli, and were regenerated into shoots. For the induction of suspended cells, the calli were transferred into a liquid MS medium containing 2mg/l NAA on a rotary shaker at 100rpm under the continuous illumination of 2, 000lux. The suspended cells developed shoot primordia through transferring into a liquid 1/2 MS basal medium containing 0.02mg/l NAA, 0.2mg/l BAP and 30g/l sucrose on a rotary shaker at 100 rpm under the continuous illumination of 2, 000lux. In order to proliferate the shoot primordia, they were transferred into a liquid B5 medium containing 1.0mg/l NAA and 0.5mg/l BAP on a gyratory shaker at 2rpm under 10, 000lux illuminated continuously by a halogen lamp. The shoot primordia regenerated plantlets on a B5 agar medium containing 0.2mg/l BAP and 10g/l sucrose. Chromosomes of suspended cells and the shoot primordia cells were examined. Seventeen percent of the suspended cells examined showed 2n=6 chromosomes with normal karyotype, and the others were karyologically abnormal. The masses of shoot primordia induced from the suspended cells were composed respectively of cells with normal, structurally changed, or duplicated karyotype. It was confirmed that the respective masses of shoot primordia were able to maintain clonally as masses of homogeneous cells with respective karyotype.
