Linkage of the Dao-1 gene for D-amino-acid oxidase to the pgm-1 gene for phosphoglucomutase-1 on the mouse chromosome 5

Abstract

Linkage of the Dao-1 gene controlling D-amino-acid oxidase to the Pgm-1 gene controlling phosphoglucomutase-1 on the mouse chromosome 5 was examined. Mutant ddY/DAO^- mice carrying a null Dao-1^c allele had a Pgm-1^a allele. Mutant mice were crossed to C3H/HeN and NZB/Kl mice carrying the Dao-1⁺ and Pgm-1^b alleles. The hybrid F₁ mice were backcrossed to the ddY/DAO^- and their progeny were examined for alleles for D-amino-acid oxidase and phosphoglucomutase-1. In both backcrosses, the progeny with recombinant-type combinations of the alleles were significantly less than the progeny with the parental-type combinations, indicating the linkage of the Dao-1 and Pgm-1 genes. The recombination frequency between these loci was estimated to be 19.8±4.0%.