The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 64, Issue 5
Displaying 1-6 of 6 articles from this issue
  • Ryuichi KONNO, Akira NIWA, Yosihiro YASUMURA
    1989 Volume 64 Issue 5 Pages 341-345
    Published: 1989
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Linkage of the Dao-1 gene controlling D-amino-acid oxidase to the Pgm-1 gene controlling phosphoglucomutase-1 on the mouse chromosome 5 was examined. Mutant ddY/DAO- mice carrying a null Dao-1c allele had a Pgm-1a allele. Mutant mice were crossed to C3H/HeN and NZB/Kl mice carrying the Dao-1+ and Pgm-1b alleles. The hybrid F1 mice were backcrossed to the ddY/DAO- and their progeny were examined for alleles for D-amino-acid oxidase and phosphoglucomutase-1. In both backcrosses, the progeny with recombinant-type combinations of the alleles were significantly less than the progeny with the parental-type combinations, indicating the linkage of the Dao-1 and Pgm-1 genes. The recombination frequency between these loci was estimated to be 19.8±4.0%.
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  • Yoshihisa FUJIO, Julie M. MACARANAS
    1989 Volume 64 Issue 5 Pages 347-354
    Published: 1989
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Malate dehydrogenase (MDH) was examined by starch gel electrophoresis for strain differences in the guppy, Poecilia reticulata. The electrophoretic patterns revealed duplicate gene loci encoding a mitochondrial form of MDH and the existence of a null allele at one of the duplicate loci. Different phenotypic frequencies at Mdh-4 were observed among 9 strains and between males and females within each strain. The progeny tests for Mdh-4 variants in the inter-strain crosses indicated a mode of partial sex-linked inheritance.
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  • Soryu NISHIBAYASHI, Yasuyuki HAYASHI, Junko KYOZUKA, Ko SHIMAMOTO
    1989 Volume 64 Issue 5 Pages 355-361
    Published: 1989
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Rice chromosomes were observed in protoplast-derived calli of two cultivars (japonica), and in plants regenerated from the calli of five cultivars (japonica). The chromosomal behavior was different between the two cultivars. In cv ‘Nipponbare’, most cells observed were stable in a diploid state (2n=24) during protoplast culture. In cv ‘Iwaimochi’, on the contrary, diploid, tetraploid, aneuploid and octoploid cells were present chimerically in calli. It is suggested that the appearance of polyploid cells in cv ‘Iwaimochi’, might have resulted in endopolyploidy during protoplast culture. In the forty-three regenerants observed in the four cultivars including ‘Nipponbare’ forty-two were diploids, while in cv ‘Iwaimochi’ tetraploid plants were regenerated at a higher frequency (6/11 plants), compared to the other four cultivars (1/43 plant). Therefore, the high frequency of tetraploid regenerants seems to correlate with the chimeric presence of diploid and tetraploid cells in calli.
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  • Toshinobu MORIKAWA
    1989 Volume 64 Issue 5 Pages 363-371
    Published: 1989
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    The genetic analysis on dwarfness of 14 accessions of wild oats, Avena fatua was carried out. They are ecotypes adapted to the man-made habitats of barley and wheat fields as a weed in East Asia. The crosses between the dwarfaccessions and the cultivated oats ′Kanota′ were made to obtain the F1 and F2 hybrids. In order to clarify the genic segregation on plant height, the frequency distribution was studied in the F2 populations of all the cross combinations. Of 14 dwarf accessions studied, 7 were controlled by a dominant gene, and 3 by a recessive gene. Two accessions showed digenic inheritance, whereas an accession (No. 288) showed polygenic inheritance and another accession (No. 342) was controlled by a incomplete dominant gene. The dwarfness in the 14 accessions was controlled by all kinds of the major genes except No. 288. Their allelic relationship is not known yet, except for the recessive gene in 3 accessions. The gene No. 812 is allelic to that of No. 169 but not to that of No. 153.
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  • Takashi TOMITA, Yoshihito WADA
    1989 Volume 64 Issue 5 Pages 373-382
    Published: 1989
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Geographic variation in sex-determining factors was studied in 19 localities in Japan between 1981 and 1986. The association of the male-determining factor, M, was demonstrated with all linkage groups, and the most predominant carrier of the M in each locality was either the IIIM or the Y chromosome. The fraction of Y in M-carrying chromosomes was higher in Hokkaido, and declined significantly on moving to the south. The frequency of the females carrying the female-determining factor, F, which is epistatic to theMfactor, was sporadically distributed in the populations surveyed. However, male heterogamety was largely retained in the populations in Hokkaido. We present a hypothesis that broadly ranging geographic variation in sex-determining factors reflects a transient state caused by recent invasions of autosomal sex-determining factors. No significant difference in the index combined with segregation rate of theM-carrying gamete and viability was indicated between the males ofX /Yand IIIM/III.
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  • Takashi TOMITA, Yoshito WADA
    1989 Volume 64 Issue 5 Pages 383-389
    Published: 1989
    Released on J-STAGE: July 25, 2006
    JOURNAL FREE ACCESS
    Housefly populations in Hokkaido, Japan were examined. The frequency of females carrying a female-determining factor (F), which is epistatic to the male-determining factor (M), has been estimated to be very low. This means that most of the chromosomes carrying an M factor are transferred from male parents to their male progeny without recombination. Based on this characteristic situation, the association between theMfactor and one of the 6PGD alleles in Hokkaido in 1983 and 1984 was studied, and strong linkage disequilibrium was found in the samples from six spots in and around Sapporo. A specific allele, 6PGD1, was associated in most of the cases with theMfactor-carrying third chromosome (IIIM). The 6PGD locus was highly polymorphic in the M factor-free third chromosome (III). Based on these experimental results, it is suggested that the frequency of chromosome IIIMhas recently increased in the population around Sapporo.
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