Abstract
Feed samples have been extracted by two digestion methods in order to prepare cell wall materials with low nitrogen contents. Two digestion methods involved extraction with neutral detergent and pronase. Furthermore the pretreatment method for removal of starch from feed samples was investigated with using α-amylase. Feed samples using in this experiment were as follows ; rice straw, orchardgrass, alfalfa, soybean meal, corn grain, wheat bran, defatted rice bran and corn silage. Pronase digestion showed higher rates of removal of total crude protein than that of neutral detergent digestion.
