Abstract
YAMAMOTO, S., T. KONDO and Y. MIND (1980) Extraction of total nonstructural carbohydrates from forage plants containing starch by α-amylase. J. Japan. Grassl. Sci. 26, 305-310. The extraction of total nonstructural carbohydrates (TNC) from forage plants containing starch was tried using α-and β-amylases. Materials tested were corn ear, alfalfa root, Rhodesgrass leaf sheath and corn starch. Non-structural carbohydrates (NC) were extracted more effectively by α-amylase (Bacillus subtilis origin) than by β-amylase (seed of Glycine hispida origin) treatment. A trace of xylose was detected only in the extract of Rhodesgrass by α-amylase treatment. The all residues treated with α-amylase retained a trace amount of NC. Alpha-amylase treatment was considered to be effectual for the extraction of TNC from forage plants.
