Abstract
We have established an efficient and stable in vitro culturing system for bahiagrass to proliferate highly regenerative embryogenic callus on modified medium increasing the level of cupric sulfate (CuSO_4). Highly regenerative embryogenic callus was initiated from shoot tips of germinated seeds and embryogenic callus was maintained on MS medium supplemented with 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/l 6-benzylaminopurine (BAP), 50μM CuSO_4, at 31℃ under the dim light condition. This modified culture could produce polyembryogenic callus which formed multiple pro-embryos over the surface of callus and gave rise to regenerability. Also, they can be maintained for more than a year without a marked loss in regenerability and appearance of albino plants during long term of culture.
