Abstract
Ti was biologically modified with photoreactive gelatin in order to regulate the attachment of cells. Photoreactive gelatin, which was synthesized by the coupling reaction of gelatin with N-(4-azidobenzoyloxy) succiminide, was immobilized onto Ti surface by ultraviolet (UV) irradiation. With the photo mask in place, micropatterning of the gelatin was performed. This pattern was confirmed by optical microscopy and time-of-flight secondary ion-mass spectroscopy (TOF-SIMS). Monkey COS-7 epithelial cells and mouse STO fibroblast cells were cultured on the pattern-immobilized plate. A significantly higher degree of cell attachment was found on the photoreactive gelatin-immobilized regions than on those that were not immobilized.
