Collagen biosynthesis in a periosteal cell pellet culture system.

Mari Akiyama; Masaaki Nakamura

doi:10.14832/gsjsdmd.2007f.0.208.0

50th General Session of the Japanese Society for Dental Materials and Devices in conjunction with International Dental Materials Congress 2007

Session ID : P-165

DOI https://doi.org/10.14832/gsjsdmd.2007f.0.208.0

Conference information

Host: The Japanese Society for Dental Materials and Devices

Poster Presentation

Collagen biosynthesis in a periosteal cell pellet culture system.

*Mari Akiyama, Masaaki Nakamura

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Keywords: bone regeneration, pellet culture, periosteal cell

CONFERENCE PROCEEDINGS FREE ACCESS

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Abstract

The present study examines collagen biosynthesis in a bovine periosteal cell pellet culture system using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Under ascorbic acid conditions, expression of both collagen type 1 and procollagen type 1 in the supernatants was observed. These results showed that procollagen type 1 was converted to collagen type 1 and that bone regeneration was dependent on vigorous collagen biosynthesis.

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