Glass beads, possibly belonging to Ainu Culture, were analyzed by XRF technique to understand the origin of these glass. The samples were excavated from Hokkaido belonging to the periods from the mid 14th to the 19th century AD. The analytical results showed that the beads can be classified into two types, lead-potash silicate glass and potash lime silica glass, based on the major components. The proportion of the potash lime silica glass in total glass analyzed is significantly higher than that usually found in beads excavated in Honshu area indicating an apparent difference in the source of the glass. A compositional similarity was observed in green potash lead silica glass between present samples and Ichijyodani Asakura Clan Ruins(15,16th century AD). Potash lime silica glass beads were classified into two groups based on trace heavy elements compositions, indicating the presence of two origins.