任意酸素濃度制御システムの改善と間歇性低酸素環境下におけるニワトリ胚の体動解析

抄録

The purpose of this study is as follows 1) to improve an arbitrary oxygen control system for an intermittent low oxygen condition with high accuracy, and 2) to investigate the characteristic body movements of the chick embryo under the intermittent hypoxic (18%) environment. The improved system controlled the oxygen concentration within error of 0.11%. The one embryo was directly recorded for 72-144 hours by a CCD camera. Although the template matching method was applied to the detection of the embryonic body movements, plenty of detection errors occurred because maintaining suitable focus on the embryo was difficult due to embryonic development in long-term measurements. The automatic focus adjusting system for the long-term measurements and more experimental data are required in the future study.