酸化損傷塩基の特異的認識を目指したアデノシン誘導体を含むオリゴヌクレオチドの合成および機能評価

抄録

The selective detection of 8-oxo-2'-deoxyguanosine (8-oxo-dG) in DNA without chemical or enzymatic treatment is an attractive tool for genomic research. We designed and synthesized the non-natural nucleoside analog, the adenosine-1,3-diazaphenoxazine derivative (Adap), for selective recognition of 8-oxo-dG in DNA. This study has clearly shown that Adap has a highly selective stabilizing effect of the duplex having the Adap-8-oxodG base pair. Furthermore, the fluorescent property of Adap has been shown to be useful for the selective detection of 8-oxodG in the duplex DNA. To the best of our knowledge, this is the first successful demonstration of the non-natural nucleoside with a high selectivity for 8-oxo-dG in DNA.