鼠癩化学療法剤のScreening Testに関する基礎的実験 其の1
鼠癩菌株の選択竝に接種部位と菌量の検討
1955 年 24 巻 3 号 p. 156-165
詳細
1955 年 24 巻 3 号 p. 156-165
It is most desirable for the screening test of chemical agents to abbreviate the experiment process and to provide the simple standards to judge the results. The following experiments were carried out to find out the murine bacillus strain, inoculation site and dosis which can satisfy those conditions. All the experiments were done with mice.
1) Bacillus Strain: By subcutaneous inoculation, the virulences of Kumamoto, Hawaiian, Keishicho and Fukuoka strains were compared.
The results presented that Fukuoka strain is weaker, and the other three strains have the similar pathogenity. (Tab. 1, 2, 3, 4)
2) Inoculation Site: Subcutaneous inoculation produced leproma at the site in an early period which can be observed exactly. (Tab. 1, 2)
Intraperitoneal and intravenous injections give a rise to the disease in various organs after long while. The predilected organ can not be found and the results are shown by bacillus distribution index and not by a practical standard. (Tab. 5, 6, 7, 8)
3) Inoculation Dosis : 0.2 ml of 102-104 diluted leproma suspension gives a rise to the disease 2 or 3 months after subctaneous inoculation. When the suspension decreases to 106-108, the longer period is necessary to produce the disease.
By intraperitoneal injection, 0.5 ml of ×50 diluted suspension produced the disease after 3-4 months. 3 months after intravenous injection 0.2ml of 102 suspension gives a rise to visible changes in the 30% of the viscera. It will take longer period to recognize lesions in all cases.
The above results ead us to conclude that the most adequate experiment is to be carried out with Hawaiian strain on account of its international value by subcutaneous inoculation in the dosis of 0.2ml of 102-104 diluted suspension, and to observe the development for 3 months.
