1955 Volume 24 Issue 6 Pages 405-415
This paper reports the simplest and the most exact judgement of the evolution of murine leprosy and selection of the adequate strain of mice after the subcutaneous inoculation.
The standards of judgement generally adapted up to now are valuation such as 1) the size of local leproma (weight an spread), 2) micro-and macroscopical changes in the lymph nodes and viscera and 3) distribution of bacilli in the various organs. As the results of our experiments, we aknowledged a proportional relation between the spread of local leproma and its weight, distribution of bacilli and the degrees of other changes. Therefore, when the spread is measured, the other two standards such as 2) and 3) can be neglected. The methods are presented in photopictures and tables.
Spread of leproma mark index 1-10mm2 ⊥ 1 11-30mm2 + 2 31-70mm ++ 3 71-mm2 +++4
The experimental strains of mice are the following 10 strains, such as NA1, NA2, S417 (Osaka Junkeidobutsu Kenkyusho), DD (Takeda Pharmaceutical Institute), dbr, DBA, S (National Institute of Genetics), B (Nagoya University), SM (Tokyo Chuo Jikkendobutsu Kenkyusho) and non-pure strain (Gifu-ken, Japan). The highest sensitivity to the murine leprosy and the smallest individual variation are recognized in B strain, (GEN. CCSSaaBB, BW _??_ 16.23g±0.61, _??_ 17.02g±0.40, CHA. Black, small strain). The rests are dbr, S, S417, DD, SM, NA1, NA2, DBA in turn. But, nowadays, the large production of B strain animal is impossible and the mortality of dbr, S, S417 is high, therefore, we are obliged to employ NA1 and NA2 mice which have lower mortality and can be produced easily.
