A METHOD FOR THE CALCULATION OF LEPROSY BACILLI AND NUMBERS OF LEPROSY BACILLI CONTAINED IN VARIOUS ORGANS CALCULATED BY THIS METHOD Kikuwo HAYASHI
1959 Volume 28 Issue 1-2 Pages 13-16
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1959 Volume 28 Issue 1-2 Pages 13-16
For the study of leprosy bacilli, especially for the decision of their proliferation, a simple but precise method for the calculation of the bacilli is considered indispensable.In this connection, a method was devised availing the proportional calculation between number of E. coli and that of leprosy bacilli.Numbers of leprosy bacilli (both human and murine) contained in various organs, nodules, and Mitsuda antigen calculated by this method were as follows: 1. Subcutaneous nodules of mice and rats contained 2×1010-4×1010/g bacilli. Generally, the nodules of mice possessed a greater number of the bacilli per gram. 2. Number of leprosy bacilli contained in human nodules was 2×109-6×109/g. 3. Batches of Mitsuda antigen prepared by the National Institute for Leprosy Research during the year 1957-1958 were found to possess nearly the same number of the bacilli the count being 2×108/cc.
