1961 Volume 30 Issue 3-4 Pages 151-158
The selection of laproma for test material of sufficiently active bacilli and a revision of Ito and Sonoda' s collection method for a purer form of the bacillary emulsion were studied for the experiments of the metabolic investigation of murine leprosy bacillus.
The results are summarized below.
1) Leproma in the rat produced 2-3 months after the intratesticular inoculation is most suitable for collecting fresh, active and viable bacilli for metabolic experiments on the marine leprosy bacillus.
2) For testing the purity of the bacillary collection, tissue components without affinity for methylene blue should be detected by dyeing with Nile blue, while some tissue components not stained with Nile blue should be demonstrated by determining of adenosine monophosphatase activity.
3) The Nile blue staining tissue debris in the bacillary fraction can be removed by repeated washing and low speed centrifugation, while the minute particles which show adenosine monophosphatase activity can be taken away be treating with 1/8 N NaOH.
