らい患者における血漿リポプロティン•リパーゼ(いわゆる清浄因子)について

抄録

The plasma lipoprotein lipase has been studied in 4 normal individuals and 26 leprosy patients, of whom 10 were of tuberculoid type, 14 were lepromatous type and 2 were borderline group, by the Stork-Kucerova's method. Ten ml of the control (pre-heparin) and 10 minute post-heparin blood samples were obtained from all subjects after an overnight fast respectively. Heparin sodium, 50mg in 5ml injected intravenously, was used as a clearing factor stimulus. The plasma was separated by centrifugation in a refrigerated centrifuge as soon as possible after withdrawal. The activity of plasma lipoprotein lipase was determined by the reduction in turbidity-measured as a fall in optical density-of the mixture of pre-or post-heparin plasma and diluted milk emulsion contained 0.03 per cent of fat. Turbidity was measured in a spectrophotometer at 610mμ after inoculation of the mixture for 2 hours at 37°C.
In tuberculoid leprosy, the degree of clearing activity extend from 12 to 34 per cent, and the average was 21.5 per cent. No difference was found in the enzyme activity of tuberculoid cases compared to that of the healthy persons. Although the activities were considerably lower in lepromatous and borderline leprosy than those of tuberculoid leprosy. Namely, the enzyme activity of lepromatous type was from 3.5 to 29.5 per cent, and the mean was 13 per cent, and that of borderline group was around 9.5 per cent. In 3 cases who had had recurrent attacks of erythema nodosum leprosum there was a moderate decrease of the enzyme activity. Electrophoretically, in most of the cases, the slight decrease in the mobility of plasma albumin was observed after intravenous administration of heparin irrespectively of age, sex or leprosy types.