Multiplication of Mycobacterium lepraemurium in cell-free liquid medium
I. Establishment of the NC medium
1973 Volume 42 Issue 2 Pages 47-51
Details
1973 Volume 42 Issue 2 Pages 47-51
It is presented evidence indicating the growth of M, lepraemurium in cell-free liquid medium of enriched Kirchner (EK) containing a-ketoglutaric acid and cytochrome C (NC). When the bacilli, Hawaiian strain, which were smeared on a silicon coated slide, were cultivated in the NC medium, the bacilli gradually elongated and multiplied. After 60 days' cultivation at 30°C, the growth of the bacilli was recognized macroscopically as a turbid mass. On the other hand, no growth was recognized in the EK medium, which is the most suitable for the growth of usual acid-fast bacilli, settled as the control, under the NC medium.
Therefore, it could be concluded that the growth of acid-fast bacilli observed in the NC medium was unequivocally the growth of M. lepraemurium.
