Multiplication of Mycobacterium lepraemurium in cell-free liquid medium
II The growth curve of M, lepraemurium in the NC medium and the pathogenicity of the bacilli grown in the medium
1973 Volume 42 Issue 2 Pages 52-55
Details
1973 Volume 42 Issue 2 Pages 52-55
In the previous paper, microscopic as well as macroscopic multiplication patterns of M. lepraemurium in cell-free liquid wedium mhich was composed of enriched Kirchner medium added with a-ketoglutaric acid and cytochrome C (NC medium) were described. In order to determine as to whether the remarkable growth patterns observed in the NC medium are due to quantitative multiplication or not, bacillary counting was periodically performed.
The results obtained indicated that the number of bacilli in the NC medium lineally increased after 10 days' latent period (lag phase), and reached the maximum at the 50th day after cultivation. The increase rate and a possible generation time, calculated from the growth curve obtained were approximately 8 times comparing with the initial in-oculum and 12.3 days, respectively.
In addition, it was noted the pathogenicity of the bacilli cultivated for 60 days in the NC medium to mice was indicated, whereas no pathogenicity of the bacilli incubated in the EK medium was shown.
