Multiplication of Mycobacterium lepraemurium in cell-free liquid medium
4. Establishment of the NC-3 medium
1973 Volume 42 Issue 3 Pages 161-164
Details
1973 Volume 42 Issue 3 Pages 161-164
It is described here that a liquid medium which is referred to as NC-3, is more satisfactory for the growth of M. lepraemurium than that of NC medium which was reported previously. After inoculating the bacilli smeared on a silicon slide glass in the NC-3 medium, the bacialli gradually elongated and multiplied. These findings are re- producible. The NC-3 medium is composed of enriched Kirchner medium added with a-ketoglutaric acid, cytochrome C and hemin.
