Repura

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Factors affecting the growth of M. lepraemurium in the NC medium were studied and the following results were obtained.
1. Possible essential substance involved in the NC medium for the growth of M. lepraemurium is a-ketoglutaric acid which is replaceable by oxaloacetate.
2. It is noted the optimal temperature for cultivation is 30°C rather than 37°C, and the culture is carried out under a slight anaerobic condition.
3. It is noted that an optimal pH of the basal medium is somewhat alkali and the serum to be used is goat one. On the contrary, acidic basal medium, horse or rat serum are inadequate for the growth of the bacilli.
4. It is demonstrated that the degree of the growth of the bacilli also depended upon the inoculum size on the meshcement coated slide; heavy inoculum resulted the bad growth pattern, and that the growth of the bacilli were stimulated by transfer of the smear to newly prepared medium at an appropriate interval.

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It is described here that a liquid medium which is referred to as NC-3, is more satisfactory for the growth of M. lepraemurium than that of NC medium which was reported previously. After inoculating the bacilli smeared on a silicon slide glass in the NC-3 medium, the bacialli gradually elongated and multiplied. These findings are re- producible. The NC-3 medium is composed of enriched Kirchner medium added with a-ketoglutaric acid, cytochrome C and hemin.

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