培養抗酸菌の精製DNA導入によるらい菌培養

抄録

Since all of catalase, peroxidase and cytochromes a and c have not been detected in Mycobacterium leprae, the cultivation of this pathogenic acid-fast strain is presumed to be impossible especially under aerobic condition. In this reason the supplementation of these defected enzymes was planned by introducing DNAs from cultivable acid-fast strains, which have all of these defected enzyme activities. As a model experiment, the introduction of SM resistant DNA into SM sensitive M. smegmatis was examined to verify the transformation. High voltage cell processor, freezing and thawing, and that combined with contusion shock were mutually compared in introducing DNA from cracks becterial cell wall, because the formation of a spheroplast was unsuccessful in M. leprae.
All the experimental results were negative. Many negative results have been seen in transformation of mycobacteria. Still more Mizuguchi also said that no genetic expression was detected in purified DNA transformation, even though positive in bacterial mating system such that arginine and methionine requirement could recover by the mating. Mycobacteria may destroy the purified DNA introduced from outside. There-fore, it was presumed that the purified DNA should be introduced to the recipient ba-cillus after integrated to bacteriophage DNA. Just then, at the 23 th U. S.-Japan Joint Conference Leprosy Panel in 1988 Jacobs reported that kanamycin resistant DNA which was inserted into the DNA of lysogenic bacteriophage L1 was expressed in M. smegmatis 607 strain.