1997 年 22 巻 1 号 p. 2-7
To produce hydrogen from sewage, aerobic evolution of hydrogen by Enterobacter aerogenes st. E.82005 was examined at the condition of unsuitable nutrient and pH for growth. E. aerogenes evolved hydrogen for more than 40 minutes under aerobic condition, DO concentration ca. 5.8mg/l. It used dissolved oxygen immediately after inoculation, though it had been cultivated under anaerobic condition. From this result, the TCA cycle and electron-transport chain were fund to be preserved even under anaerobic condition. The termination of hydrogen evolution at aerobic condition was explained as the oxidation of NADH turned from hydrogen evolution to the respiration.