Notes on naturalized plants in Hiroshima Prefecture: 4. New locality and phylogeny of Sisymbrium orientale L. (Brassicaceae) and its related species

Abstract

Naturalized plants, Sisymbrium orientale L. and S. irio L. (Brassicaceae) were collected from Hiroshima Prefecture, Honshu, southwestern Japan. The former species was found growing by the road-side at reclamation areas in Mihara, Higashihiroshima and Hatsukaichi Cities as additional localities in the prefecture; the latter was found by the road-side i n Hiroshima City a nd is the fi rst record i n the prefecture. Sequence of the nuclear 18S rRNA gene (nr 18S rDNA) and ribosomal internal transcribed spacer (nr ITS), and chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene (cp rbcL) for S. orientale; nr ITS for S. irio and S. officinale were obtained. Their phylogenetic positions were also demonstrated.