Abstract
Global transcriptional profiles of the yeast Saccharomyces cerevisiae were studied following changes in growth conditions to high hydrostatic pressure (25 MPa, 24 °C) and low temperature (0.1 MPa, 15 °C). These profiles were quantitatively very similar. Particularly, expression of the DAN/TIR cell wall mannoprotein genes, which are generally expressed under hypoxia, were markedly upregulated by high pressure and low temperature, suggesting the overlapping regulatory networks of transcription. In support of the role of the mannoproteins in cell wall integrity, cells acquired resistance against treatment with low concentrations of SDS and Zymolyase, and lethal level of high pressure (125 MPa) when cells were preincubated under high pressure and low temperature.
