2008 Volume 2 Issue 1 Pages 29-37
Three-dimensional structures of glucose isomerase molecules in crystals prepared from supersaturated solutions in capillaries under 0.1 and 100 MPa were determined by X-ray crystallography under 0.1 MPa. With accurate temperature control, we obtained a few large crystals of good quality in the capillaries under 0.1 and 100 MPa. X-ray structures with high quality data sets were successfully obtained without removing solution around the crystals. We also determined a relation between resolution of molecular structure and size of the crystals surrounded by the solution in the capillaries. However, the r.m.s. deviation of least square fit of all atom positions of both glucose isomerase folds was about 0.05Å. This deviation was little. The distributions of water sites in both glucose isomerase structures were almost identical.
