International Chemical Engineering Symposia Proceedings
Online ISSN : 2758-3031
IChES (International Chemical Engineering Symposia) 2022
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Temperature Effect on the Separation Performance of Chromatography
Shinya NOZAKINoriko YOSHIMOTOShuichi YAMAMOTO
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CONFERENCE PROCEEDINGS OPEN ACCESS

Pages 13-17

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Abstract

The effect of temperature T on the chromatographic separation performance was examined by using a model separation system: polyphenol isocratic elution separation by polymer-resin chromatography with ethanol-water mobile phases within the temperature range 15-45°C. HETP decreased with increasing T. The reduced HETP and the reduced velocity plots can be approximated by a single universal curve. The equations describing the distribution coefficient K as a function of T were also developed. A simple equation was derived, which can describe the resolution of two components Rs as a function of T. Rs did not increase significantly with T while the separation time can be reduced.

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This is an open access article published under a CC BY-NC-ND license.
https://creativecommons.org/licenses/by-nc-nd/4.0/
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